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| Registros recuperados: 22 | |
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| Souza,Paula Monteiro de; Bittencourt,Mona Lisa de Assis; Caprara,Carolina Canielles; Freitas,Marcela de; Almeida,Renata Paula Coppini de; Silveira,Dâmaris; Fonseca,Yris Maria; Ferreira Filho,Edivaldo Ximenes; Pessoa Junior,Adalberto; Magalhães,Pérola Oliveira. |
| Proteases hydrolyze the peptide bonds of proteins into peptides and amino acids, being found in all living organisms, and are essential for cell growth and differentiation. Proteolytic enzymes have potential application in a wide number of industrial processes such as food, laundry detergent and pharmaceutical. Proteases from microbial sources have dominated applications in industrial sectors. Fungal proteases are used for hydrolyzing protein and other components of soy beans and wheat in soy sauce production. Proteases can be produced in large quantities in a short time by established methods of fermentation. The parameters such as variation in C/N ratio, presence of some sugars, besides several other physical factors are important in the development of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proteases; Enzyme production; Fungal protease; Industrial application. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200337 |
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| Cunha,Rodrigo L. O. R.; Gouvea,Iuri E.; Juliano,Luiz. |
| Tellurium is a rare element which has been regarded as a toxic, non-essential trace element and its biological role is not clearly established to date. Besides of that, the biological effects of elemental tellurium and some of its inorganic and organic derivatives have been studied, leading to a set of interesting and promising applications. As an example, it can be highlighted the uses of alkali-metal tellurites and tellurates in microbiology, the antioxidant effects of organotellurides and diorganoditellurides and the immunomodulatory effects of the non-toxic inorganic tellurane, named AS-101, and the plethora of its uses. Inasmuch, the nascent applications of organic telluranes (organotelluranes) as protease inhibitors and its applications in disease... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antitumorals; Biological applications; Enzyme inhibitors; Organotellurium; Proteases; Tellurium. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300006 |
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| Tiago,Patrícia Vieira; Silva,Ricardo José da. |
| Cigarrinhas são as principais pragas da pastagem e da cana-de-açúcar, destacando-se os gêneros Deois e Mahanarva. Essas pragas vêm causando sérios danos a estas culturas na região de Tangará da Serra, MT, Brasil. O fungo Metarhizium anisopliae é considerado um eficiente agente para o controle biológico de insetos, possuindo vários fatores de virulência, incluindo a produção de proteases, consideradas fundamentais no processo de penetração através da cutícula do inseto. Este trabalho tem como objetivos analisar a produção de proteases extracelulares a partir de isolados de M. anisopliae, utilizando substratos cuticulares e não-cuticulares, e verificar a influência desses substratos na expressão dessas enzimas. Foram utilizados 4 isolados de M. anisopliae... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungos entomopatogênicos; Proteases; Mahanarva fimbriolata; Deois flavopicta. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000100005 |
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| Frías-Quintana,Carlos Alfonso; Álvarez-González,Carlos Alfonso; Guerrero-Zárate,Rocío; Valverde-Chavarría,Silvia; Ulloa-Rojas,Juan B.. |
| ABSTRACT Wolf cichlid, Parachromis dovii, is a species with a high potential for aquaculture in Central America; however, the knowledge of the digestive physiology in larvae period is limited. For these reason, this study evaluated the changes on digestive enzymes (alkaline and acid proteases, trypsin, chymotrypsin, aminopeptidase, carboxypeptidase, lipases, amylases, and phosphatases) during early ontogeny by biochemical analysis. All digestive enzymes were detected at first feeding (6 days after hatching, DAH, 9.49 mm, 168 degree-days DD). Afterwards all enzymes reached two main peaks in activity at 14 or 22 DAH (15.10 mm, 364 DD and 20.83 mm, 550 DD, respectively). Later, there was a gradual decrease in activity for trypsin and acid and alkaline... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Larviculture; Proteases; Zymogram. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100215 |
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| Frías-Quintana,Carlos Alfonso; Peña-Marín,Emyr Saul; Ramírez-Custodio,Carlos David; Martínez-García,Rafael; Jiménez-Martínez,Luis Daniel; Camarillo-Coop,Susana; Guerrero-Zárate,Rocío; Asencio-Alcudia,Gloria Gertrudys; Álvarez-González,Carlos Alfonso. |
| ABSTRACT In the Southeast of Mexico, there are many native cichlids with commercial interest such as redhead cichlid (Vieja melanurus) and twoband cichlid (V. bifasciata), which have a great local demand and excellent meat quality. However, it is necessary to implement their culture based on nutrition studies and digestive biochemistry. This study’s objective was to characterize these two cichlids’ digestive proteases (pH, temperature, and inhibitors) through biochemistry techniques. Results showed that V. melanurus and V. bifasciata have a digestive capacity analogous to other omnivore fishes, where the optimal pH values of stomach proteases (4 and 2, respectively) and intestinal proteases (6 and 12, respectively), the optimal temperature of acid (35°C... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inhibitors; PH; Proteases; Stability; Temperature. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100210 |
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| Staats,Charley Christian; Boldo,Juliano; Broetto,Leonardo; Vainstein,Marilene; Schrank,Augusto. |
| Mycoplasmas are very fastidious in their nutritional requirements for in vitro growth and have limited biosynthetic capacity, a reflection of their reduced genomes. As a result, these bacteria depend upon external metabolites for nutrition and growth and have developed dependence on their hosts for survival and maintenance. Protein degradation and peptide importation play an important role in Mycoplasma spp. nutrition, and proteases can play a role in host adaptation and pathogenicity. Here, we present a general survey on the genes involved in protein degradation, secretion and importation, comparing all available Mollicute genomes. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; Proteases; Minimal genomes; Secretion systems. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200009 |
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| Bordusa,F.. |
| This article reports on the design and characteristics of substrate mimetics in protease-catalyzed reactions. Firstly, the basis of protease-catalyzed peptide synthesis and the general advantages of substrate mimetics over common acyl donor components are described. The binding behavior of these artificial substrates and the mechanism of catalysis are further discussed on the basis of hydrolysis, acyl transfer, protein-ligand docking, and molecular dynamics studies on the trypsin model. The general validity of the substrate mimetic concept is illustrated by the expansion of this strategy to trypsin-like, glutamic acid-specific, and hydrophobic amino acid-specific proteases. Finally, opportunities for the combination of the substrate mimetic strategy with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioorganic chemistry; Enzyme catalysis; Peptide synthesis; Proteases; Substrate mimetics. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500001 |
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| Merino-Contreras,María L.; Sánchez-Morales,Froylán; Jiménez-Badillo,María L.; Peña-Marín,Emyr S.; Álvarez-González,Carlos A.. |
| ABSTRACT Digestive proteases were partially characterized in sheepshead juveniles, using biochemical and electrophoretic techniques. Results showed higher activity level of the stomach proteases (2.39 ± 0.02 U mg protein-1) compared to the intestinal proteases (1.6 ± 0.1 U mg protein-1). The activity of trypsin, chymotrypsin, leucine aminopeptidase and carboxypeptidase A was also recorded. The optimum temperature of the stomach proteases was recorded at 45 °C, while for intestinal proteases was recorded at 55 °C. Stomach proteases showed less stability to temperature changes than intestinal proteases. An optimum pH of 2 was recorded for stomach proteases with high stability under acidic conditions, while an optimum pH of 9 was recorded for intestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; Inhibitors; Pepsin; Proteases; Zymogram. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000400206 |
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| Guzmán,Fanny; Barberis,Sonia; Illanes,Andrés. |
| Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis... |
| Tipo: Journal article |
Palavras-chave: Enzymatic synthesis; Peptides; Proteases; Solid-phase synthesis. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012 |
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| Amorim,Fernanda Gobbi; Longhim,Heloisa Tavoni; Cologna,Camila Takeno; Degueldre,Michel; Pauw,Edwin De; Quinton,Loïc; Arantes,Eliane Candiani. |
| Abstract Background: Tityus serrulatus venom (Ts venom) is a complex mixture of several compounds with biotechnological and therapeutical potentials, which highlights the importance of the identification and characterization of these components. Although a considerable number of studies have been dedicated to the characterization of this complex cocktail, there is still a limitation of knowledge concerning its venom composition. Most of Ts venom studies aim to isolate and characterize their neurotoxins, which are small, basic proteins and are eluted with high buffer concentrations on cation exchange chromatography. The first and largest fraction from carboxymethyl cellulose-52 (CMC-52) chromatography of Ts venom, named fraction I (Fr I), is a mixture of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tityus serrulatus; Scorpion venom; Enzymes; Proteases; ACE inhibitors; Proteome. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100308 |
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| Cordeiro,Francielle Almeida; Coutinho,Bárbara Marques; Wiezel,Gisele Adriano; Bordon,Karla de Castro Figueiredo; Bregge-Silva,Cristiane; Rosa-Garzon,Nathalia Gonsales; Cabral,Hamilton; Ueberheide,Beatrix; Arantes,Eliane Candiani. |
| Abstract Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV). Methods and results: Lmr-MP was purified through two chromatographic steps and submitted to enzymatic characterization. It showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lachesis muta rhombeata; Metalloprotease; Proteases; Snake venom. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100323 |
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| Boechat,L.H.B.; Zollner,R.L.. |
| Studies concerning the antigenicity of thyroglobulin fragments allow the characterization of the epitopes but do not consider the role of heavier antigenic fragments that could result in vivo from the action of endoproteases. Here we assess the relative importance of the fragments obtained from thyroglobulin by limited proteolysis with trypsin and compare by immunoblotting their reactivity to serum from patients with autoimmune (Graves' disease and Hashimoto's thyroiditis) and non-autoimmune (subacute thyroiditis) disease. The results showed no difference in frequency of recognition of any peptide by sera from patients with autoimmune thyroiditis. In contrast, sera from patients with subacute thyroiditis reacted more frequently with a peptide of 80 kDa.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autoantibodies; Autoimmunity; Thyroglobulin; Trypsin; Thyroiditis; Proteases. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400012 |
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| Registros recuperados: 22 | |
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